This document details an example of the pipetting precision and accuracy data for four specified volumes across a full 96 well plate.

Experimental design

• Precision and accuracy were tested by separately measuring the volume of 96 transfers of 50 μL, 100 μL, 500 μL and 1000 μL
• Water was dispensed sequentially (8 channels simultaneously) starting with column 1 (positions A1-H1) and ending with column 12 (positions A12-H12)
• Performance was assessed by measuring the weights of 96 collection tubes before and after the addition of purified water
• Volume data was generated for each position using temperature corrected density values

Because of the low volumes and weights involved, correction factors for temperature and evaporation rate were applied: