This document details an example of the pipetting precision and accuracy data for four specified volumes across 24 (4 x 6) positions.

Experimental design

• Precision and accuracy were tested by separately measuring the volume of 24 transfers of 50 uL, 100 uL, 500 uL and 1000 uL
• Water was dispensed sequentially (4 channels simultaneously) starting with column 1 (positions A1-D1) and ending with column 6 (positions A6-D6)
• Performance was assessed by measuring the weights of 24 collection tubes before and after the addition of purified water
• Volume data was generated for each position using temperature corrected density values

Because of the low volumes and weights involved, correction factors for temperature and evaporation rate were applied.