Comparison of Biotage Extrahera vs manual sample processing using a vacuum manifold

Automated sample preparation using the Biotage Extrahera was compared to an equivalent manual method utilizing a vacuum manifold. A selection of beta blocker drugs were extracted from pooled stripped plasma using a supported liquid extraction procedure. ISOLUTE SLE+ 200 μL sample volume plates, part number 820-0200-P01 were used for extraction. Resulting extracts from both preparation methods were subsequently analyzed by LC-MS/MS.