Comparison of Biotage Extrahera vs. manual sample processing using a vacuum manifold

Automated sample preparation using the Biotage Extrahera was compared to an equivalent manual method utilizing a vacuum manifold. Analytes were extracted from pooled stripped plasma using a supported liquid extraction procedure. ISOLUTE SLE+ 400 uL sample volume plates, part number 820-0400-P01 were used for extraction. Resulting extracts from both sample preparation methods were subsequently analyzed by LC-MS/MS