Extraction of nicotine and metabolites from urine, serum, plasma and whole blood using ISOLUTE SLE+ prior to LC-MS/MS analysis

Author(s)

Biotage

ISOLUTE SLE+ products provide clean, rapid, robust, efficient, high throughput and automatable extraction solutions for a wide range of analytes.

This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of nicotine, cotinine, 3-OH-cotinine, nornicotine, norcotinine and anabasine from urine, serum, plasma and whole blood using ISOLUTE SLE+ plates with LC-MS/MS detection.