ISOLUTE SLE+ supported liquid extraction columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and significantly reduced sample preparation time.

This application note describes the supported liquid extraction clean-up of a wide range of opiates and metabolites from urine prior to quantitative GC-MS analysis. An effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant opiates and their metabolites is demonstrated.