Sample preparation and protein: a comparison of protein removal with various sample preparation techniques using gel electrophoresis

Author(s)

Biotage

This poster evaluates the effectiveness of various sample preparation techniques for the removal of serum protein. By performing 1-D-gel electrophoresis on the extracts it was possible to obtain protein profiles for the various techniques.

The techniques investigated:

Protein precipitation
Supported liquid extraction (SLE+)
Non-polar silica-based SPE
Non-polar polymer-based SPE
Silica-based mixed-mode SPE (anion and cation exchange)
Polymer-based mixed-mode cation exchange SPE