Symmetric Flow FFF is the premier technique for the analysis of complex and/or sticky macromolecules such as polysaccharides, proteins and aggregates, which cannot be characterized by conventional chromatographic methods. Furthermore, FFF can separate the components of the conjugated protein sample, as well as provide hydrodynamic diameter information regarding the proteins, protein-polysaccharide conjugates and aggregates in a single run. By using FFF, a complete overview of the sample can be obtained quickly.