In this study, asymmetrical flow FFF (AF4) was used to separate a mixture of human serum albumin - binding 63Cu and transferrin - binding 56Fe metal ions. The AF4 system separated the proteins by size and detected the metal ions by ICP-MS to obtain metal distributions of the protein mixture. The system setup consisted of Postnova AF2000 MT and Agilent 7700 ICP-MS.
Hyphenation of asymmetrical flow field-flow fractionation (AF4) and ICP-MS for multi-element analysis and characterization of plasma proteins