Amino acid composition of protein was analyzed by DAB-Label which is a kit for pre-column derivatization amino acid analysis with DABS-Cl (Dimethylamino azobenzene-sulfonyl chloride).

1 ug of Myoglobin was vapor-phase hydrolyzed with hydrochloric acid (6N HCl, 110 ̊C, 24 hrs) and derivatized with DABS-Cl. The analysis of the derivatives was performed by HPLC.